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R&D Systems human il 33 duoset elisa kit

A CellChat analysis of human lung snRNA-seq data. B Venn of 47 shared DEGs from four datasets ( GSE159354 , GSE132771 , GSE128033 , GSE122960 ). C Heatmap of 47-gene expression across cell types. D Correlation analysis (Pearson Correlation) of expression of IGFBP5, IL33, MGP, SPRY1, ACKR1 and mechanical stress scores in ECs. E Correlation analysis (Pearson Correlation) of expression of PIEZO1 and IGFBP5, IL33, MGP, SPRY1, ACKR1 in ECs. F Human snRNA-seq UMAP and violin of IL33 distribution and subpopulations. G Bar chart showed expression of IL33 (mean ± SEM) in IPF (n = 7966 cells) vs control (n = 4174 cells). P value was calculated using the two-tailed unpaired t-test. H Violin of IL33 in PIEZO1 - vs PIEZO1 + ECs. I Bulk-RNA-seq of saline and BLM (7 days and 14 days) induced mice (n = 3 per group). Gene expression levels were Z-score normalized. P value was calculated using the two-tailed unpaired t-test. J qPCR of Il33 in BLM mouse lung ECs. <t>K</t> <t>IL-33</t> (green) and CD31 (red) co-staining in human IPF vs normal, quantification is shown at right (n = 10). L IL-33 (green) and CD31 (red) co-staining in Yoda1- or GsMTx4-treated fibrotic mouse lungs, quantification is shown at right (n = 3). M IL-33 (green) and CD31 (red)co-staining in Piezo1 WT and Piezo1 ΔEC BLM lungs, quantification is shown at right (n = 3). Error bars represent mean ± SEM, H, K, J, M: two-tailed unpaired t-test; L: one-way ANOVA followed Tukey’s multiple comparwasons test, All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Human Il 33 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 78 article reviews

human il 33 duoset elisa kit - by Bioz Stars, 2026-09

94/100 stars

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1) Product Images from "Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis"

Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis

Journal: Nature Communications

doi: 10.1038/s41467-026-70193-w

Figure Legend Snippet: A CellChat analysis of human lung snRNA-seq data. B Venn of 47 shared DEGs from four datasets ( GSE159354 , GSE132771 , GSE128033 , GSE122960 ). C Heatmap of 47-gene expression across cell types. D Correlation analysis (Pearson Correlation) of expression of IGFBP5, IL33, MGP, SPRY1, ACKR1 and mechanical stress scores in ECs. E Correlation analysis (Pearson Correlation) of expression of PIEZO1 and IGFBP5, IL33, MGP, SPRY1, ACKR1 in ECs. F Human snRNA-seq UMAP and violin of IL33 distribution and subpopulations. G Bar chart showed expression of IL33 (mean ± SEM) in IPF (n = 7966 cells) vs control (n = 4174 cells). P value was calculated using the two-tailed unpaired t-test. H Violin of IL33 in PIEZO1 - vs PIEZO1 + ECs. I Bulk-RNA-seq of saline and BLM (7 days and 14 days) induced mice (n = 3 per group). Gene expression levels were Z-score normalized. P value was calculated using the two-tailed unpaired t-test. J qPCR of Il33 in BLM mouse lung ECs. K IL-33 (green) and CD31 (red) co-staining in human IPF vs normal, quantification is shown at right (n = 10). L IL-33 (green) and CD31 (red) co-staining in Yoda1- or GsMTx4-treated fibrotic mouse lungs, quantification is shown at right (n = 3). M IL-33 (green) and CD31 (red)co-staining in Piezo1 WT and Piezo1 ΔEC BLM lungs, quantification is shown at right (n = 3). Error bars represent mean ± SEM, H, K, J, M: two-tailed unpaired t-test; L: one-way ANOVA followed Tukey’s multiple comparwasons test, All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Techniques Used: Gene Expression, Expressing, Control, Two Tailed Test, RNA Sequencing, Saline, Staining

A Schematic of BLM-induced fibrosis in Il33 WT vs Il33 ΔEC mice. B Il33 mRNA (n = 4) and IL-33 protein in lung ECs (n = 3). C Representative Masson/PSR images and quantification (n = 5). D αSMA immunofluorescence and quantification (n = 4). E Lung hydroxyproline content (n = 5). F Schematic: EC-specific Il33 overexpression via AAV-Tie1 in Piezo1 WT and Piezo1 ΔEC mice; 4-week pretreatment, BLM at week 4, sacrifice at week 7. G IL-33 expression in ECs, immune and epithelial cells after AAV-OE- Il33 (n = 3). H Masson/PSR images and quantification (n = 5). I αSMA immunofluorescence and quantification (n = 4). J Hydroxyproline content (n = 5). Error bars: mean ± SEM; B,G: two-tailed unpaired t-test; C,D-E,I: two-way ANOVA followed Tukey’s multiple comparisons test; H,J: three-way ANOVA followed Tukey’s multiple comparisons test. All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Figure Legend Snippet: A Schematic of BLM-induced fibrosis in Il33 WT vs Il33 ΔEC mice. B Il33 mRNA (n = 4) and IL-33 protein in lung ECs (n = 3). C Representative Masson/PSR images and quantification (n = 5). D αSMA immunofluorescence and quantification (n = 4). E Lung hydroxyproline content (n = 5). F Schematic: EC-specific Il33 overexpression via AAV-Tie1 in Piezo1 WT and Piezo1 ΔEC mice; 4-week pretreatment, BLM at week 4, sacrifice at week 7. G IL-33 expression in ECs, immune and epithelial cells after AAV-OE- Il33 (n = 3). H Masson/PSR images and quantification (n = 5). I αSMA immunofluorescence and quantification (n = 4). J Hydroxyproline content (n = 5). Error bars: mean ± SEM; B,G: two-tailed unpaired t-test; C,D-E,I: two-way ANOVA followed Tukey’s multiple comparisons test; H,J: three-way ANOVA followed Tukey’s multiple comparisons test. All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Techniques Used: Immunofluorescence, Over Expression, Expressing, Two Tailed Test

HUVECs under 20% stretch for 0,6,24 h or 2,12,25 kPa substrates; IL-33 secretion ( n = 3) A and mRNA (n = 3) B . C PIEZO1 knock-down with sh PIEZO1 ( n = 3). HUVECs were transduced with sh PIEZO1 for 48 h, cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch before measuring IL-33 secretion ( n = 3) D and mRNA ( n = 3) E . HUVECs were cultured on 2, 12, 25 kPa substrates for 24 h or stretched (20%) for 0, 6, 24 h, followed by measurement of calpain activity F and CAPN2 protein G ( n = 3). HUVECs (sh PIEZO1 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, followed by measurement of calpain activity ( n = 3) H and CAPN2 protein levels ( n = 3) I . J CAPN2 knock-down with shCAPN2 ( n = 3). K HUVECs (sh CAPN2 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, IL-33 secretion(left) and mRNA(right) ( n = 3). L Model for paracrine IL-33 regulation. M Cistrome: STAT3 predicted to drive IL-33 regulation. N intersection of top 100 motifs in BLM-treated mouse ECs with top 10 TFs. O The protein levels of P-STAT3 and STAT3 in HUVECs treated with 20% stretch for 0 and 6 hours ( n = 3). P The protein levels of P-STAT3 and STAT3 in HUVECs (sh PIEZO1 or sh CAPN2 ) treated with 20% stretch for 6 h ( n = 3). Q STAT3 knock-down with sh STAT3 ( n = 3). R HUVECs (sh STAT3 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch; IL-33 secretion(left) and mRNA(right) ( n = 3). Error bars: mean ± SEM; C , G , J , O – Q : two-tailed unpaired t-test; A , B , F : one-way ANOVA followed Šídák’s multiple comparisons test, D – E , H – I , K , R : two-way ANOVA Tukey’s multiple comparisons test. All n denote biologically independent samples. Source data are provided as a Source Data file.

Figure Legend Snippet: HUVECs under 20% stretch for 0,6,24 h or 2,12,25 kPa substrates; IL-33 secretion ( n = 3) A and mRNA (n = 3) B . C PIEZO1 knock-down with sh PIEZO1 ( n = 3). HUVECs were transduced with sh PIEZO1 for 48 h, cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch before measuring IL-33 secretion ( n = 3) D and mRNA ( n = 3) E . HUVECs were cultured on 2, 12, 25 kPa substrates for 24 h or stretched (20%) for 0, 6, 24 h, followed by measurement of calpain activity F and CAPN2 protein G ( n = 3). HUVECs (sh PIEZO1 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, followed by measurement of calpain activity ( n = 3) H and CAPN2 protein levels ( n = 3) I . J CAPN2 knock-down with shCAPN2 ( n = 3). K HUVECs (sh CAPN2 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, IL-33 secretion(left) and mRNA(right) ( n = 3). L Model for paracrine IL-33 regulation. M Cistrome: STAT3 predicted to drive IL-33 regulation. N intersection of top 100 motifs in BLM-treated mouse ECs with top 10 TFs. O The protein levels of P-STAT3 and STAT3 in HUVECs treated with 20% stretch for 0 and 6 hours ( n = 3). P The protein levels of P-STAT3 and STAT3 in HUVECs (sh PIEZO1 or sh CAPN2 ) treated with 20% stretch for 6 h ( n = 3). Q STAT3 knock-down with sh STAT3 ( n = 3). R HUVECs (sh STAT3 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch; IL-33 secretion(left) and mRNA(right) ( n = 3). Error bars: mean ± SEM; C , G , J , O – Q : two-tailed unpaired t-test; A , B , F : one-way ANOVA followed Šídák’s multiple comparisons test, D – E , H – I , K , R : two-way ANOVA Tukey’s multiple comparisons test. All n denote biologically independent samples. Source data are provided as a Source Data file.

Techniques Used: Knockdown, Transduction, Cell Culture, Activity Assay, Two Tailed Test

Enzyme-linked Immunosorbent Assay:

Article Title: The TAK1→IKKβ→TPL2→MKK1/MKK2 Signaling Cascade Regulates IL-33 Expression in Cystic Fibrosis Airway Epithelial Cells Following Infection by Pseudomonas aeruginosa.
Article Snippet: .. IL-33 protein levels were measured by ELISA with the human IL-33 DuoSet Elisa kit (R&D systems catDY3625-05). .. Results were analyzed with GraphPad Prism software (version 5.0), p-values less than 0.05 were considered to be significant.

Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis
Article Snippet: The primary antibodies used were CD31 (Abcam, #ab9498; Servicebio, #GB13063), VE-cadherin (R&D Systems, #AF938), IL-33 (R&D Systems, #AF3626), PIEZO1(proteintech, #15939-1-AP) and αSMA (Abcam, #ab7817) antibodies. .. The amounts of IL-33 in HUVECs supernatant were quantified using the Human IL-33 DuoSet ELISA kit (R&D Systems, #D3300B) according to the manufacturer’s instructions. .. Absorbance was read on a SpectraMax Plus 384 full-spectrum microplate reader (Molecular Devices) and the data were analyzed with OriginPro 9.1.

Article Title: Interleukin-33 Signaling Controls the Development of Iron-Recycling Macrophages
Article Snippet: Cell analysis was done using BD FACSDiva v8.01 Software and figure displayed dot plots and histograms were obtained using FlowJo v10.5 software (FlowJo, LLC). .. We used MesoScale Discovery IL-33 V-Plex assay kit (product code K152XBD-1) and human IL-33 Duoset ELISA kit (DY3625, R&D systems) according to manufacturer’s instructions. ..

Article Title: Chinese medicine Yu-Ping-Feng-San attenuates allergic inflammation by regulating epithelial derived pro-allergic cytokines
Article Snippet: [ABSTRACT] This study aimed to investigate the mechanisms of Yu-Ping-Feng-San (YPFS) on attenuating allergic inflammation in the initial stage of atopic dermatitis (AD).. AD mouse model was established with fluorescein isothiocyanate (FITC) sensitization and elicitation.. Epithelial barrier structure was observed with transmission electron microscope.

Article Title: Platelet and IL-33 Count as Biomarkers for Lung Function Impairment: An 11-Year Follow-Up Study on Populations Exposed to Hexavalent Chromium.
Article Snippet: Recent research has highlighted the crucial role of immune regulation in lung function impairment due to exposure to hazardous materials.. This study aimed to identify dynamic network biomarkers for lung function damage caused by hexavalent chromium inhalation exposure, using immune-related indicators in blood.. An 11year follow-up longitudinal study was conducted on a population occupationally exposed to hexavalent chromate (Cr [VI]) from 2010 to 2020, consisting of sixty-one subjects with 328 repeat measurements.

Article Title: IL-33 stimulates the release of procoagulant microvesicles from human monocytes and differentially increases tissue factor in human monocyte subsets
Article Snippet: Stefan Stojkovic1; Åsa Thulin2; Lena Hell3; Barbara Thaler1; Sabine Rauscher4; Johanna Baumgartner1; Marion Gröger4; Cihan Ay3; Svitlana Demyanets5; Christoph Neumayer6; Ihor Huk6; Andreas Spittler4,6; Kurt Huber7,8; Johann Wojta1,4,8,9; Agneta Siegbahn2; Mikael Åberg2 1Department of Internal Medicine II, Medical University of Vienna, Austria; 2Department of Medical Sciences, Clinical Chemistry and Science for Life Laboratory, Uppsala University, Uppsala, Sweden; 3Department of Internal Medicine I, Clinical Division of Hematology and Hemostaseology, Medical University of Vienna, Austria; 4Core Facilities, Medical University of Vienna, Austria; 5Department of Laboratory Medicine, Medical University of Vienna, Austria; 6Department of Surgery, Division of Vascular Surgery, Medical University of Vienna, Austria; 73rd Medical Department for Cardiology and Emergency Medicine, Wilhelminen Hospital, Vienna, Austria; 8Sigmund FReud Private University, Medical School, Vienna, Austria; 9Ludwig Boltzmann Cluster for Cardiovascular Research, Vienna Austria

Article Title: Higher interleukin-33 levels in aggressive periodontitis cases
Article Snippet: .. IL-33 concentration in GCF and plasma, obtained from the study subjects, were measured using Human IL-33 DuoSet ELISA kit (R and D Systems, USA, Imported by Biotech-India, India) and estimated using the standard curve, in accordance with the instructions provided in the kit insert. ..

Article Title: The TAK1→IKKβ→TPL2→MKK1/MKK2 Signaling Cascade Regulates IL-33 Expression in Cystic Fibrosis Airway Epithelial Cells Following Infection by Pseudomonas aeruginosa
Article Snippet: .. IL-33 protein levels were measured by ELISA with the human IL-33 DuoSet Elisa kit (R&D systems catDY3625-05). .. Results were analyzed with GraphPad Prism software (version 5.0), p -values less than 0.05 were considered to be significant.

Clinical Proteomics:

Recombinant:

Concentration Assay:

Image Search Results

Journal: Nature Communications

Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis

doi: 10.1038/s41467-026-70193-w

Figure Lengend Snippet: A CellChat analysis of human lung snRNA-seq data. B Venn of 47 shared DEGs from four datasets ( GSE159354 , GSE132771 , GSE128033 , GSE122960 ). C Heatmap of 47-gene expression across cell types. D Correlation analysis (Pearson Correlation) of expression of IGFBP5, IL33, MGP, SPRY1, ACKR1 and mechanical stress scores in ECs. E Correlation analysis (Pearson Correlation) of expression of PIEZO1 and IGFBP5, IL33, MGP, SPRY1, ACKR1 in ECs. F Human snRNA-seq UMAP and violin of IL33 distribution and subpopulations. G Bar chart showed expression of IL33 (mean ± SEM) in IPF (n = 7966 cells) vs control (n = 4174 cells). P value was calculated using the two-tailed unpaired t-test. H Violin of IL33 in PIEZO1 - vs PIEZO1 + ECs. I Bulk-RNA-seq of saline and BLM (7 days and 14 days) induced mice (n = 3 per group). Gene expression levels were Z-score normalized. P value was calculated using the two-tailed unpaired t-test. J qPCR of Il33 in BLM mouse lung ECs. K IL-33 (green) and CD31 (red) co-staining in human IPF vs normal, quantification is shown at right (n = 10). L IL-33 (green) and CD31 (red) co-staining in Yoda1- or GsMTx4-treated fibrotic mouse lungs, quantification is shown at right (n = 3). M IL-33 (green) and CD31 (red)co-staining in Piezo1 WT and Piezo1 ΔEC BLM lungs, quantification is shown at right (n = 3). Error bars represent mean ± SEM, H, K, J, M: two-tailed unpaired t-test; L: one-way ANOVA followed Tukey’s multiple comparwasons test, All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Article Snippet: The amounts of IL-33 in HUVECs supernatant were quantified using the Human IL-33 DuoSet ELISA kit (R&D Systems, #D3300B) according to the manufacturer’s instructions.

Techniques: Gene Expression, Expressing, Control, Two Tailed Test, RNA Sequencing, Saline, Staining

Journal: Nature Communications

Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis

doi: 10.1038/s41467-026-70193-w

Figure Lengend Snippet: A Schematic of BLM-induced fibrosis in Il33 WT vs Il33 ΔEC mice. B Il33 mRNA (n = 4) and IL-33 protein in lung ECs (n = 3). C Representative Masson/PSR images and quantification (n = 5). D αSMA immunofluorescence and quantification (n = 4). E Lung hydroxyproline content (n = 5). F Schematic: EC-specific Il33 overexpression via AAV-Tie1 in Piezo1 WT and Piezo1 ΔEC mice; 4-week pretreatment, BLM at week 4, sacrifice at week 7. G IL-33 expression in ECs, immune and epithelial cells after AAV-OE- Il33 (n = 3). H Masson/PSR images and quantification (n = 5). I αSMA immunofluorescence and quantification (n = 4). J Hydroxyproline content (n = 5). Error bars: mean ± SEM; B,G: two-tailed unpaired t-test; C,D-E,I: two-way ANOVA followed Tukey’s multiple comparisons test; H,J: three-way ANOVA followed Tukey’s multiple comparisons test. All n values indicate biologically independent samples. Source data are provided as a Source Data file.

Article Snippet: The amounts of IL-33 in HUVECs supernatant were quantified using the Human IL-33 DuoSet ELISA kit (R&D Systems, #D3300B) according to the manufacturer’s instructions.

Techniques: Immunofluorescence, Over Expression, Expressing, Two Tailed Test

Journal: Nature Communications

Article Title: Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis

doi: 10.1038/s41467-026-70193-w

Figure Lengend Snippet: HUVECs under 20% stretch for 0,6,24 h or 2,12,25 kPa substrates; IL-33 secretion ( n = 3) A and mRNA (n = 3) B . C PIEZO1 knock-down with sh PIEZO1 ( n = 3). HUVECs were transduced with sh PIEZO1 for 48 h, cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch before measuring IL-33 secretion ( n = 3) D and mRNA ( n = 3) E . HUVECs were cultured on 2, 12, 25 kPa substrates for 24 h or stretched (20%) for 0, 6, 24 h, followed by measurement of calpain activity F and CAPN2 protein G ( n = 3). HUVECs (sh PIEZO1 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, followed by measurement of calpain activity ( n = 3) H and CAPN2 protein levels ( n = 3) I . J CAPN2 knock-down with shCAPN2 ( n = 3). K HUVECs (sh CAPN2 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch, IL-33 secretion(left) and mRNA(right) ( n = 3). L Model for paracrine IL-33 regulation. M Cistrome: STAT3 predicted to drive IL-33 regulation. N intersection of top 100 motifs in BLM-treated mouse ECs with top 10 TFs. O The protein levels of P-STAT3 and STAT3 in HUVECs treated with 20% stretch for 0 and 6 hours ( n = 3). P The protein levels of P-STAT3 and STAT3 in HUVECs (sh PIEZO1 or sh CAPN2 ) treated with 20% stretch for 6 h ( n = 3). Q STAT3 knock-down with sh STAT3 ( n = 3). R HUVECs (sh STAT3 ) were cultured on 25 kPa substrates for 24 h or subjected to 6 h mechanical stretch; IL-33 secretion(left) and mRNA(right) ( n = 3). Error bars: mean ± SEM; C , G , J , O – Q : two-tailed unpaired t-test; A , B , F : one-way ANOVA followed Šídák’s multiple comparisons test, D – E , H – I , K , R : two-way ANOVA Tukey’s multiple comparisons test. All n denote biologically independent samples. Source data are provided as a Source Data file.

Article Snippet: The amounts of IL-33 in HUVECs supernatant were quantified using the Human IL-33 DuoSet ELISA kit (R&D Systems, #D3300B) according to the manufacturer’s instructions.

Techniques: Knockdown, Transduction, Cell Culture, Activity Assay, Two Tailed Test

Group 2 innate lymphoid cells are increased in the blood of male and female Veterans with asthma in comparison to healthy sex-matched controls. (A-G) A representative flow gating strategy is shown for identifying the CD127+ CRTH2+ ILC2. (H) . The percentage of the total viable CD45+ cells that are CRTH2+ ILC2 was determined by flow cytometry. (I) . The count of CRTH2+ ILC2 per mL of blood collected are shown. (J-O) Cytokine detection in plasma by ELISA. (J) IL-5, (K) IL-13 (L) IL-17 (M) TSLP, (N) IL-33, and (O) Amphiregulin (Areg). Statistical analysis was performed using a One-way ANOVA with a Kruskal-Wallis post-test. N=30 with asthma, N=32 healthy. Statistical difference between groups is shown as * p < 0.05. Exact p-value included for statistical trend between 0.05 and 0.1.

Journal: Frontiers in Immunology

Article Title: Serum 17 β -estradiol inversely correlates with circulating group 2 innate lymphoid cells in a cohort of asthmatic patients

doi: 10.3389/fimmu.2025.1555228

Figure Lengend Snippet: Group 2 innate lymphoid cells are increased in the blood of male and female Veterans with asthma in comparison to healthy sex-matched controls. (A-G) A representative flow gating strategy is shown for identifying the CD127+ CRTH2+ ILC2. (H) . The percentage of the total viable CD45+ cells that are CRTH2+ ILC2 was determined by flow cytometry. (I) . The count of CRTH2+ ILC2 per mL of blood collected are shown. (J-O) Cytokine detection in plasma by ELISA. (J) IL-5, (K) IL-13 (L) IL-17 (M) TSLP, (N) IL-33, and (O) Amphiregulin (Areg). Statistical analysis was performed using a One-way ANOVA with a Kruskal-Wallis post-test. N=30 with asthma, N=32 healthy. Statistical difference between groups is shown as * p < 0.05. Exact p-value included for statistical trend between 0.05 and 0.1.

Article Snippet: Plasma was analyzed for human IL-5 (cat# DY205), IL-8 (cat# DY208), IL-10 (cat# DY217), IL-13 (cat# DY213), IL-17 (cat# DY317), Amphiregulin (Areg) (cat# DY262) and IL-33 (cat# DY3626) using the Duoset ELISA kits from R&D Systems.

Techniques: Comparison, Flow Cytometry, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Estrogen inversely correlates with ILC2 numbers in circulation regardless of biologic sex or asthma status. (A, B) Serum was processed from whole blood collected from each donor and extracted prior to hormone level determination. (A) Progesterone and (B) estrogen levels were determined after extraction using ELISA. Donor data was compiled for all study participants, where complete data was available subject data was correlated with (C) ILC2/mL of blood collected and (D) the % of ILC2 per total CD45+ cells by simple linear regression, and (E) a Pearson’s correlation matrix for hormones, BMI and immune populations. * indicates a p-value < 0.05, *** p < 0.001.

Journal: Frontiers in Immunology

Article Title: Serum 17 β -estradiol inversely correlates with circulating group 2 innate lymphoid cells in a cohort of asthmatic patients

doi: 10.3389/fimmu.2025.1555228

Figure Lengend Snippet: Estrogen inversely correlates with ILC2 numbers in circulation regardless of biologic sex or asthma status. (A, B) Serum was processed from whole blood collected from each donor and extracted prior to hormone level determination. (A) Progesterone and (B) estrogen levels were determined after extraction using ELISA. Donor data was compiled for all study participants, where complete data was available subject data was correlated with (C) ILC2/mL of blood collected and (D) the % of ILC2 per total CD45+ cells by simple linear regression, and (E) a Pearson’s correlation matrix for hormones, BMI and immune populations. * indicates a p-value < 0.05, *** p < 0.001.

Techniques: Extraction, Enzyme-linked Immunosorbent Assay

Fig. 3 Human nasal commensalS. epidermidispromoted the reduction of IL-33 production in the nasal epithelium. ARNE cells from four AR sub jects were inoculated with human nasal commensal, S. epidermidis at a multiplicity of infection (MOI) of 0.25. Levels of mRNAs encoding epithelial cell-de rived cytokines including interleukin including IL-33 (A) and GATA3 (B) which is transcription factor involved in Th2 cell differentiation were monitored by real-time PCR. Results are presented as mean ± SD values from four independent experiments. *p < 0.05 vs. mock-infected ARNE cells. Wild type mice (PBS/ PBS) and AR mice (OVA/OVA) were inoculated with human nasal S. epidermidis (3.2 × 106 CFU/30 µl PBS) at indicated time points. The mRNA expression of IL-33 (C) was measured by real-time PCR and IL-33 protein concentration (D) secreted from nasal mucosa were measured by ELISA using nasal lavage fluid. The mRNA expression of GATA3 (E) was also measured using real-time PCR. Results are presented as mean ± standard deviation (SD) (N = 5). *p < 0.05

Journal: BMC microbiology

Article Title: Symbiotic microbiome Staphylococcus epidermidis restricts IL-33 production in allergic nasal epithelium via limiting the cellular necroptosis.

doi: 10.1186/s12866-023-02898-7

Figure Lengend Snippet: Fig. 3 Human nasal commensalS. epidermidispromoted the reduction of IL-33 production in the nasal epithelium. ARNE cells from four AR sub jects were inoculated with human nasal commensal, S. epidermidis at a multiplicity of infection (MOI) of 0.25. Levels of mRNAs encoding epithelial cell-de rived cytokines including interleukin including IL-33 (A) and GATA3 (B) which is transcription factor involved in Th2 cell differentiation were monitored by real-time PCR. Results are presented as mean ± SD values from four independent experiments. *p < 0.05 vs. mock-infected ARNE cells. Wild type mice (PBS/ PBS) and AR mice (OVA/OVA) were inoculated with human nasal S. epidermidis (3.2 × 106 CFU/30 µl PBS) at indicated time points. The mRNA expression of IL-33 (C) was measured by real-time PCR and IL-33 protein concentration (D) secreted from nasal mucosa were measured by ELISA using nasal lavage fluid. The mRNA expression of GATA3 (E) was also measured using real-time PCR. Results are presented as mean ± standard deviation (SD) (N = 5). *p < 0.05

Article Snippet: Secreted human IL-33 (DY3625B) and mouse IL-33 (DY3626) were quantified using the DuoSet® ELISA kit from R&D Systems according to the manufacturer’s instructions.

Techniques: Infection, Cell Differentiation, Real-time Polymerase Chain Reaction, Expressing, Protein Concentration, Enzyme-linked Immunosorbent Assay, Standard Deviation

Fig. 4 S. epidermidisreduces IL-33 production via suppression of RIPK3/MLKL-dependent necroptosis and is independent of apoptosis in al lergic nasal epithelium. ARNE cells from four AR subjects were inoculated with human nasal commensal, S. epidermidis at a multiplicity of infection (MOI) of 0.25. The mRNA expression of IL-33 (A) was measured by real-time PCR, IL-33 protein concentration (B) secreted from ARNE cells was measured by ELISA using supernatants, and intracellular IL-33 protein expression (C) was also measured. after treatment of necroptosis inhibitors. Key molecules of necrop tosis including RIPK3 (cropped blot) and MLKL and apoptosis including caspase3 were monitored by Western blot analysis (D). The mRNA expression of RIPK3 (E), and MLKL (F), and caspase3 (G) was measured by real-time PCR. Results are presented as mean ± SD values from four independent experiments. *p < 0.05 vs. mock-infected ARNE cells

Journal: BMC microbiology

Article Title: Symbiotic microbiome Staphylococcus epidermidis restricts IL-33 production in allergic nasal epithelium via limiting the cellular necroptosis.

doi: 10.1186/s12866-023-02898-7

Figure Lengend Snippet: Fig. 4 S. epidermidisreduces IL-33 production via suppression of RIPK3/MLKL-dependent necroptosis and is independent of apoptosis in al lergic nasal epithelium. ARNE cells from four AR subjects were inoculated with human nasal commensal, S. epidermidis at a multiplicity of infection (MOI) of 0.25. The mRNA expression of IL-33 (A) was measured by real-time PCR, IL-33 protein concentration (B) secreted from ARNE cells was measured by ELISA using supernatants, and intracellular IL-33 protein expression (C) was also measured. after treatment of necroptosis inhibitors. Key molecules of necrop tosis including RIPK3 (cropped blot) and MLKL and apoptosis including caspase3 were monitored by Western blot analysis (D). The mRNA expression of RIPK3 (E), and MLKL (F), and caspase3 (G) was measured by real-time PCR. Results are presented as mean ± SD values from four independent experiments. *p < 0.05 vs. mock-infected ARNE cells

Article Snippet: Secreted human IL-33 (DY3625B) and mouse IL-33 (DY3626) were quantified using the DuoSet® ELISA kit from R&D Systems according to the manufacturer’s instructions.

Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction, Protein Concentration, Enzyme-linked Immunosorbent Assay, Western Blot

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human il 33 duoset elisa kit (R&D Systems)

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1) Product Images from "Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosis"

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